Microscopy Publications

Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis of Xenopus mesendoderm tissue at gastrulation​
by Pooja R. Sonavane, et al.

  • ABSTRACT: The coordination of individual cell behaviors is a crucial step in the assembly and morphogenesis of tissues. Xenopus mesendoderm cells migrate collectively along a fibronectin (FN) substrate at gastrulation, but how the adhesive and mechanical forces required for these movements are generated and transmitted is unclear..

Development. 144(23):4363-4376 (2017)

Microscopy Core Facilities: Results of an International Survey​
by Masilamani Elangovan, Ammasi Periasamy & Horst Wallrabe

Microscopy Today March: pp36-44 (2014)

Advanced Light Microscopy
by Ammasi Periasamy

Methods 15: 66(2):121-123 (2014)

Characterization of Two-photon Excitation Fluorescence Lifetime Imaging Microscopy for Protein Localization 
by Ye Chen & Ammasi Periasamy 

  • ABSTRACT: Two-photon excitation .uorescence resonance energy transfer (2P-FRET) imaging microscopy can provide details of speci.c protein molecule interactions inside living cells. Fluorophore molecules used for 2P-FRET imaging have characteristic absorption and emission spectra that introduce spectral cross-talk (bleed-through) in the FRET signal that should be removed in the 2P-FRET images, to establish that ...

Micoscopy Research and Technique 63:72-80 (2004)

Fluorescence Microscopy Study of Heterogeneity in Polymer-supported Luminescence-based Oxygen Sensors
by Kristi A. Kneas, et al.

  • ABSTRACT: Despite the great potential of fluorescence microscopy, its application to date has largely been in the study of biological specimens. It will be shown that conventional fluorescence microscopy provides an invaluable tool with which to study the photophysics of polymer-supported luminescence-based oxygen sensors. The design of the imaging system, the measurement methods, and the data analysis used in the investigation of sensor systems are described.​..

Microscopy and Microanalysis. 6: 551-556 (2000) 

An Evaluation of Two-Photon Excitation Versus Confocal and Digital Deconvolution Fluoescence Microscopy Imaging in Xenopus Morphogenesis 
by Ammasi Periasamy, et al.

  • ABSTRACT: The ability to visualize cell motility occurring deep in the context of opaque tissues will allow many currently intractable issues in developmental biology and organogenesis to be addressed. In this study, we compare two-photon excitation with laser scanning confocal and conventional digital deconvolution fluorescence microscopy, using the same optical configuration, for their ability to resolve cell shape deep in Xenopus gastrula and neurula tissues....

Microscopy Research and Technique 47:172-181 (1999) 

High-Speed Fluorescence Microscopy: Lifetime Imaging in the Biomedical Sciences
by Ammasi Periasamy, et al.

  • ABSTRACT: The ability to observe the behavior of living cells and tissues provides unparalleled access to information regarding the organization and dynamics of complex cellular structures. While great strides have been made over the past 30 to 40 years in the design and application of a variety of novel optical microscopic techniques, until recently, it has not been possible to image biological phenomena that occur over very short time periods (nanosecond to millisecond) or over short distances (10 to 1000 Å)...

J. Micros. Soc. Am., 1: 13-23 (1995)

Computerized fluorescence microscopic vision in the biomedical sciences​
by Ammasi Periasamy & Brian Herman

  • ABSTRACT: The quantitative information obtainable from light-microscope images has been improved dramatically through the combination of advanced digital image processing technology, novel optical microscope techniques and highly sensitive video cameras. The ability of this instrumentation to intensify weakly fluorescent signals at the level of single living cells, without injuring the cell, has led to major advances in the understanding of the physiology and pathophysiology of living cells...

J. Comp. Assist. Micros., 6: 1-26 (1994)