Clinical Applications

Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population 
by Majid Siadat-Pajouh, et al. 

  • ABSTRACT: During gastrulation, an embryo acquires the three primordial germ layers that will give rise to all of the tissues in the body. In amniote embryos, this process occurs via an epithelial to mesenchymal transition (EMT) of epiblast cells at the primitive streak. Although the primitive streak is vital to development, many aspects of how it forms and functions remain poorly understood.​..

Dev. Dynamics 241(2):270-283 (2012) 

Angiotensin II Type 2 Receptor–Bradykinin B2 Receptor Functional Heterodimerization​
by Peter M. Abadir, et al.

  • ABSTRACT: Angiotensin II type 2 (AT2R) or bradykinin B2 (B2R) receptor activation enhances NO production. Recently, we demonstrated enhancement of NO production when AT2R and B2R are simultaneously activated in vivo. However, the mechanism involved in this enhancement is unknown. Using confocal fluorescence resonance energy transfer microscopy, we report the distance between the AT2R and B2R in PC12W cell membranes to be 50±5 Å, providing evidence and quantification of receptor heterodimerization as the mechanism for enhancing NO production.​..

Hypertension (JAHA, Journal of the American Heart Association) 48:1-7 (2006)


Journal (JBO) Special Section on MP (2003)
by Ammasi Periasamy & Alberto Diaspro


Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBPα) in the living pituitary cell nucleus​
by Richard N. Day, et al.

  • ABSTRACT: The homeodomain (HD) protein Pit-1 cooperates with the basic-leucine zipper (b-ZIP) protein CCAAT/enhancer binding protein alpha (C/EBPα) to control pituitary-specific prolactin (PRL) gene transcription. We previously observed that C/EBPα was concentrated in regions of centromeric heterochromatin in pituitary GHFT1-5 cells and that co-expressed Pit-1 re-distributed C/EBPα to the subnuclear sites occupied by Pit-1.​..

Mol. Endo. 17(3), 333-345 (2003)


Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule​
by Jonathan E. Gale, et al.

  • ABSTRACT: Our senses of hearing and balance depend upon hair cells, the sensory receptors of the inner ear. Millions of people suffer from hearing and balance deficits caused by damage to hair cells as a result of exposure to noise, aminoglycoside antibiotics, and antitumor drugs. In some species such damage can be reversed through the production of new cells. This proliferative response is limited in mammals but it has been hypothesized that damaged hair cells might survive and undergo intracellular repair​...

J. Neurobiol.50, 81-92 (2002)


Truncated Estrogen Receptor Product-1 Suppresses Estrogen Receptor Transactivation by Dimerization with Estrogen Receptors α and β​
by Eileen M. Resnick, et al.

  • ABSTRACT: The estrogen receptor (ER) is a ligand-activated transcription factor that acts as a homodimer. Truncated estrogen receptor product-1 (TERP-1) is a pituitary-specific, estrogen-induced, isoform of rat ERα that is transcribed from a unique start site and contains only the C-terminal region of the full-length receptor. TERP-1 does not affect transcription directly but suppresses ligand-activated ERα and ERβ activity.​..

J. Biol. Chem., 275: 7158-7166 (2002)


Detection Of Human Papillomavirus Type 16/18 DNA In Cervicovaginal Cells By Fluorescence Based In Situ Hybridization And Automated Image Cytometry 
by Majid Siadat-Pajouh, et al. 

  • ABSTRACT: Automatic fluorescence image cytometry (AFIC) is a fast, sensitive, and reliable approach for screening slide-based clinical specimens. In this study, we applied AFIC to identify cancer-associated human papillomavirus (HPV) genotypes...

Cytometry 15:245-257 (1994) 

Verapamil and cyclosporin A modulate doxorubicin toxicity by distinct mechanisms​
by Yoko Shoji, et al. 

  • ABSTRACT: Cyclosporin A has been reported to enhance the sensitivity of cells displaying multiple drug resistance to anthracyclines. However, the mechanism of action of cyclosporin A in modulating drug resistance is still controversial. This study compares the effects of cyclosporin A and verapamil on doxorubicin resistance in chinese hamster ovary cells (CHRC5) using several criteria including in vitro cytotoxicity, drug accumulation, intracellular distribution by video microscopy, and nuclear DNA damage.​..

Cancer Letters. 57:  209-218 (1991)