TIRF - Total Internal Reflection Fluorescence Microscopy

This is a custom built system using Olympus optics and other companies’ optics and lasers. This system is equipped with an Olympus (IX-70) Infinity corrected inverted microscope, Objective lenses: 2X, 4X/0.13, 10X/0.30, 20X/0.40, 40X/0.85 Oil, 60X/1.20 WI, 60X/1.40 Oil, 60X (TIRF, 1.45NA, Oil), 100X/1.40 Oil, Two independent Olympus TIRF illuminators allow simultanous Green & Red TIRF imaging, Argon laser ( 488 nm), DPSS laser 556 nm,  Hamamatsu high speed high sensitivity cMOS CCD camera,  Temperature controlled chamber and Eppendorf microinjection device and Metamorph Software.

TIRF – Xenopus Laevis embryonic cells. Red: actin (LifeAct- mCherry) Green: c-cadherin
Katherine Pfister, Ray Keller, Biology