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Schedule:March 10-13, 2004

March 10, 2004 , Wednesday

  
7:30 – 8:30

Registration

8:30 – 9:00 Introduction about the workshop, the staffs, and the participants
Ammasi Periasamy, Workshop Coordinator , Center Director
Departments of Biology and Biomedical Engineering
Remarks by Prof. Raymond Keller, Chair, Department of Biology
9:00 – 9:40 “Basics of Fluorescence and FRET”
James N. Demas, Department of Chemistry, UVA
9:40 – 10:10 Optical Microscopy - I
Ammasi Periasamy
10:10 – 10:30

Coffee Break (Room# 190)

10:30 – 11:00 Optical Microscopy - II
Ammasi Periasamy
11:00 – 11:30 Filters for FRET Microscopy
Michael Stanley, Chroma Technology
11:30 – 12:10 Seeing Colors: the application and limitations of the fluorescent proteins
Richard Day ,Workshop Coordinator
Departments of Cell Biology and Medicine
12:10 – 13:00 Lunch
13:00 - 17:15 Laboratory
March 11, 2004, Thursday  
8:00 – 8:20

Revision

8:20 – 9:00 GFPs in FRET microscopy
Richard Day
9:00 – 10:00 FRET Data Analysis – the Algorithm
Ammasi Periasamy
10:00 – 10:30

Coffee Break (Room# 190)

10:30 – 11:10 FRET in Membranes: Special Considerations
Anne Kenworthy, Department of Molecular Physiology and Biophysics,
Vanderbilt University School of Medicine, Tennessee
11:10 – 11:50 Basics of Fluorescence Lifetime Imaging (FLIM)
Robert M. Clegg, Laboratory for Fluorescence Dynamics
University of Illinois, Urbana-Champaign
12:00 – 1:00 Lunch
13:00 - 17:15 Laboratory
March 12, 2004, Friday  
8:00 – 8:15

Revision

8:20 - 9:00 FRET Microscopy Reveals Clustered Distribution of Co-internalized Receptor-ligand Complexes in the Apical Recycling Endosome of Polarized Epithelial MDCK cells
Margarida Barroso , Cardiovascular Research Center, Albany, NY
9:00 – 9:30 FRET Demonstrates formation of BAD/Bcl/xL Complexes in Injured Axons following Traumatic Brain Injury in Rats
James Mills / Gregory Helm,
Department of Neurosurgery
9:30 – 10:00 FRET in Alzheimer's disease
Michelle King/George Bloom, Department of Biology
10:00 – 10:30 Coffee Break
10:30 – 11:10 FRET with Single Molecules: the Dynamics of Ribozymes
Robert Clegg
11:10 – 11:50 Fluorescence Correlation Spectroscopy for Quantifying Molecular Dynamics and interactions
Keith Berland, Department of Physics, Emory University, Atlanta
12:00 - 13:00 Lunch
13:00 - 17:15 Laboratory
March 13, 2004, Saturday