Schedule:

March 5-9, 2003

March 5, 2003, Wednesday

Gilmer Room #190

12:00 – 1:30

Registration & Lunch

1:30 – 2:00

Introduction about the workshop, the staffs, and the participants
Ammasi Periasamy, Workshop Coordinator
Center Director &
Departments of Biology and Biomedical Engineering

Remarks by
Prof. Raymond Keller, Chair, Department of Biology
Prof. Jay W. Fox, Asst Dean of Research, University of Virginia Health Sciences Center

2:00 – 3:00pm

Keynote Speaker Introduction by
Richard Day, Workshop Coordinator
Departments of Medicine and Cell Biology, UVA

Keynote Lecture
“Some History and Nuts-‘n-Bolts about FRET”
Robert M. Clegg
Professor of Physics and Biophysics
Laboratory for Fluorescence Dynamics
University of Illinois, Urbana-Champaign

3:00 – 3:30

“Laser Scanning Confocal Microscopy”
Jeffrey Larson, Nikon

3:30 – 4:00

Coffee Break

4:00 – 5:00

“Basics of Fluorescence and FRET”
James N. Demas, Department of Chemistry, UVA

5:00 – 5:40

“Basics of Multiphoton Microscopy”
Peter So, Dept. of Mechanical Engineering and Biological Engineering
Massachusetts Institute of Technology, Boston

March 6, 2003, Thursday

Gilmer Room #190

7:30 – 8:00 am

Breakfast

8:00 – 8:15

Revision

8:20 – 8:50

“Microscopy and FRET Imaging”
Ammasi Periasamy

8:50 – 9:20

“Modern Imaging and Analysis methods in confocal and 2photon Microscopy”
Sebastian Tille, Carl Zeiss

9:20 – 10:00

“FRET Data Acquisition and Analysis”
(wide-field, confocal, multiphoton, and algorithm for data analysis)
Ammasi Periasamy

10:00 – 10:30

Coffee Break

10:30 – 11:20

“FRET Data Acquisition and Analysis”
(wide-field, confocal, multiphoton, and algorithm for data analysis)
Ammasi Periasamy

11:20 – 12:00

“Seeing Colors: Applications and Limitations of the Fluorescent Proteins”
Richard N. Day

12:00 – 1:00

Lunch

1:00 – 6:00

Laboratory (Rooms # 145, 148, 154 & 156)
151 – Data Analysis – Ye Chen

3:30 - 4:00

Coffee Break

March 6, 2003, Thursday

Detailed Laboratory Schedule

Room 190
1:00 – 1:10

Margarida Barroso, How to use the cells in the microscopy system

1:10 – 1:20

Richard Day, How to use the cells in the microscopy system

After small presentation about the cells for investigation, the staffs will guide the respective group to the respective microscopy room

1:20 – 4:30

Work on the respective microscopy system to collect the images for FRET data processing

4:30 – 5:00

Acceptor Photobleaching demo

4:30 – 5:00

Positive and Negative control demo

5:00 – 6:00

Vendors demo of their system to the participants

March 7, 2003, Friday

Gilmer Room #190

7:30 – 8:00 am

Breakfast

8:00 – 8:15

Revision

8:20 – 9:00

“FRET in Membranes: Special Considerations”
Anne Kenworthy, Department of Molecular Physiology and Biophysics Vanderbilt University School of Medicine, Tennessee

9:00 – 9:40

“FRET Microscopy Reveals Clustered Distribution of Co-internalized Receptor-ligand Complexes in the Apical Recycling Endosome of Polarized Epithelial MDCK cells”
Margarida Barroso, Department of Biology, UVA

9:40 – 10:10

“FLIM-FRET Microscopy”
Ammasi Periasamy

10:10 – 10:30

Coffee Break

10:30 – 11:00

“Fluorescence Correlation Spectroscopy for Quantifying Molecular Dynamics and interactions”
Keith Berland, Department of Physics
Emory University, Atlanta

11:00 – 11:30

“FRET with Single Molecules: the Dynamics of Ribozymes”
Robert Clegg

11:30 – 12:10

“Using the Fluorescent Proteins in FRET Microscopy”
Richard N. Day

12:10 – 1:00

Lunch

1:10 – 6:00

Laboratory (Rooms # 145, 148, 154 & 156)
151 – Data Analysis – Ye Chen

3:30 - 4:00

Coffee Break

March 7, 2003; Friday

Detailed Laboratory schedule

1:20 – 4:30

work on the respective microscopy system to collect the images for FRET data processing

4:30 – 5:00

Acceptor Photobleaching demo

4:30 – 5:00

Positive and Negative control demo

5:00 – 6:00

Vendors demo of their system to the participants

March 8, 2003, Saturday

Gilmer Room #190

7:30 – 8:00 am

Breakfast

8:00 – 8:15

Revision

8:20 – 9:00

“Looking for Lipid Rafts using FRET Microscopy”
Anne Kenworthy

9:00 – 9:30

“FRET Demonstrates formation of BAD/Bcl/xL Complexes in Injured Axons following Traumatic Brain Injury in Rats”
James Mills, Department of Neurosurgery, UVA

9:30 – 10:10

“Several ways for Measuring FRET in the Microscope with Live Organisms (C. elegans)”
Robert Clegg

10:10 – 10:30

Coffee Break

10:30 – 11:00

“Mathematical Modeling for Analysis of Clustering of Ligand-receptor Complexes in Endocytic Membranes”
Margarida Barroso

11:00 – 11:40

“Biological Approaches to Verifying FRET Results”
Martin Schwartz, Cardiovascular Research Center and
Department of Microbiology, UVA

11:40 – 12:10pm

Spectral Imaging Microscopy
Lee Peachey, Leica

12:10 – 1:00pm

Lunch

1:00 – 5:00

Laboratory (Rooms # 145, 148, 154 & 156)
151 – Data analysis – Ye Chen

3:30 - 4:00

Coffee Break

March 8, 2003, Saturday

Detailed Laboratory Schedule

1:00 – 3:30

work on the respective microscopy system to collect the images for data processing

3:30 – 4:00

Acceptor Photobleaching demo

3:30 – 4:00

Positive and Negative control demo

4:00 - 5:00

Vendors demo of their system to the participants

Visit to Monticello

5:00 – 6:30

Thomas Jefferson’s House

6:45 – 7:30

Social and Tour of the Michie Tavern

7:30 – 8:30

Dinner

March 9, 2003, Sunday

Gilmer Room #190

8:30 – 9:00

Breakfast

9:00 – 11:00

Discussion includes questions/clarifications about the workshop to the teaching faculty

Participant’s presentation/discussions if they have questions regarding implementation of FRET methods for their biological applications

11:00 - 12:00

Lunch

Workshop officially closed after lunch

All the Vendors should pack up their system on or before 4pm Sunday and should move their boxes out of the rooms.