The 2015 Workshop on FRET and FLIM microscopy
The W.M. Keck Center for Cellular Imaging, a University Imaging Center is sponsoring an advanced course on fluorescence resonance energy transfer (FRET) microscopy. The workshop will be an intensive lecture/laboratory series that will enable participants to develop theoretical and hands-on expertise with various FRET microscopy techniques. Attendees will closely interact with the teaching faculty and assistants while using modern research grade microscopes equipped with essential filters, objective lenses, high-sensitivity detectors (CCD cameras and PMTs), and state-of-the-art user friendly software. This four days intensive course includes morning lectures and afternoon hands on training on FRET microscopy systems. Experienced staff from universities and industry will provide instruction. With a maximum enrollment of 20 students, there will be ample opportunity to the attendees can work with various state-of-the-art FRET microscopes, video cameras, recorders, and image processing software.
Topics to be covered include:
The workshop is designed primarily for research scientists, physicians, postdoctoral trainees and graduate students in medical, animal, and plant research. Non-biologists seeking a comprehensive introduction to FRET microscopy will benefit greatly from this course as well. There are no specific prerequisites, but an understanding of the basic principles of microscopy and molecular biology is desirable.
The five-day course consists of lectures, training on various FRET microscopy, exercises, discussions, and presentation by the attendees that will enable the attendees to obtain and interpret FRET images of high quality, to understand the importance of qualitative and quantitative FRET data analysis.