TIRF

Total Internal Reflection Fluorescence (TIRF) Microscopy

  • Olympus (IX-70) Infinity corrected inverted microscope.
  • Objective lenses: 2X, 4X, 10X, 20X, 40X Oil, 60X WI, 60X Oil, 60X (TIRF, 1.45NA, Oil), 100X Oil.
  • Two independent Olympus TIRF illuminators allow simutanous Green & Red TIRF imaging.
  • Argon laser (457, 488, 514 nm), DPSS laser 556 nm, HeNe laser 633 nm.
  • Hamammatsu high speed high sensitivity cMOS CCD camera.
  • Temperature controlled chamber and Eppendorf microinjection device.
  • Metamorph Software.

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