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  1. FLIM Imaging

    or TIRF microscopy to implement FLIM or FLIM-FRET imaging. A rapid lifetime determination (RLD) method ... Fluorescence Lifetime Imaging Microscopy (FLIM) The fluorescence lifetime refers to the average ... imaging. We have both one and two-three-photon lifetime imaging systems. a.    TD FLIM: The most common ...

    keckcenter - 11/17/2015 - 16:14

  2. FRET

    wide-field,  confocal, spectral and  2p microscopy  as well as  FRET/FLIM, each with its own advantage ... What is FRET? Förster (Fluorescence) Resonance Energy Transfer (FRET) is a process involving ... be higher than or equal to the acceptor fluorophore. There are three important conditions for FRET to occur: ...

    artsci - 10/20/2017 - 15:04

  3. FRET Microscopy

    wide-field,  confocal, spectral and  2p microscopy  as well as  FRET/FLIM, each with its own advantage ... What is FRET? Förster (Fluorescence) Resonance Energy Transfer (FRET) is a process involving ... be higher than or equal to the acceptor fluorophore. There are three important conditions for FRET to occur: ...

    Content Manager - 10/20/2017 - 15:10

  4. General Information

    advanced light microscopy techniques. We run an annual workshop on FLIM & FRET microscopy during March ... lifetime imaging microscopy (FLIM) systems (3), wide-field microscopy, high content screening (HCS) system, ...

    artsci - 10/20/2017 - 16:04

  5. Periasamy, Ph.D.

    multiphoton, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization ... a hands on training Annual Workshop on FLIM & FRET Microscopy at the University of Virginia, ... fluorescence lifetime imaging microscopy (FLIM) for measuring the oscillations in cytosolic and nuclear free ...

    artsci - 07/21/2018 - 15:48

  6. User Fees

    Micrcoscopy: $40/hour   [Training fee $200- onetime fee per PI] Lifetime imaging (FLIM) and FRET: $40/hour   [Training ... fee $200- onetime fee per PI] Data Analysis Training (FRAP, FRET, FLIM, FCS, Lightsheet, ...

    artsci - 05/07/2018 - 09:32

  7. List of Publications

    Imaging Protein molecules using FRET-FLIM microscopy. Current Opinion in Biotechnology. 16:19-27. 40.      ... Excitation FRET-FLIM Microscopy. Microscopy and Microanalysis. 11(suppl 2):12-13. 41.      Chen, Y. ... Voss, T.C., Chen, Y. and Periasamy, A. (2004) FLIM-FRET microscopy to visualize transcription factor ...

    keckcenter - 07/21/2018 - 16:01

  8. FRET Pair Spectra

    FRET Pair Spectra 2-Photon Spectra ...

    keckcenter - 10/20/2017 - 15:25

  9. One-day Symposium on Microscopy Imaging at the University of Virginia

    SEM, Confocal, Spectral, Multiphoton, in vivo/intravital imaging, TIRF, FRAP, FCS, FLIM, FRET ...

    keckcenter - 09/18/2015 - 12:59

  10. Participating Vendors

    16th Annual Workshop on FLIM & FRET Microscopy W. M. Keck Center for Cellular Imaging (KCCI) ...

    keckcenter - 03/04/2017 - 10:37

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