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  1. FLIM-FRET Microscopy

    currently available fluorescence microscopic techniques, such as confocal or multi-photon excitation, cannot ... and frequency domain FLIM imaging systems are available on the market. FLIM-FRET The combination of lifetime ... and FRET (FLIM-FRET) provides high spatial (nanometer) and temporal (nanoseconds) resolution (Bacskai et ...

    keckcenter - 10/20/2017 - 15:24

  2. Multiphoton FRET Microscopy

    and depth resolution without a confocal aperture. The multiphoton technique considerably reduces ... at a wavelength substantially shorter than the excitation wavelength. How to collect FRET Images In Multiphoton ... FRET (MP-FRET) imaging, we select the appropriate filters and high sensitivity photomultiplier tubes ...

    keckcenter - 10/20/2017 - 15:23

  3. FRET Pair Spectra

    FRET Pair Spectra 2-Photon Spectra ...

    keckcenter - 10/20/2017 - 15:25

  4. Zeiss 780 NLO

    Zeiss 780 Confocal/spectral/multiphoton Imaging Microscopy System The Zeiss 780 NLO system ... consists of an inverted Axio Observer microscope, laser scanning confocal, 32 channels spectral imaging, ... the Multi-Timing Macro, Multicolor+3D+time, FRAP, FRET, tile imaging, multitrack scanning to avoid crosstalk, ...

    keckcenter - 10/20/2017 - 16:05

  5. List of Publications

    Wallrabe, H., Sun, Y., Fang, X., Periasamy A. and Bloom, G.S., (2015) Three-color confocal FRET ... of protein-protein interactions: Confocal FRET Microscopy. Meth. Cell Biol. 89: 569-598 27.      Hewang Li et al., ... of protein-protien interactions in living cells using confocal and spectral imaging FRET microscopy. Indian J Exp. ...

    keckcenter - 07/21/2018 - 16:01

  6. FLIM Imaging

    photon confocal microcopy, such as less photobleaching, photodamage, scattering for live cell and tissue ... laser scanning microscopes both confocal and multi-photon mode with high speed detectors ... fluorophores such as used for FRET or endogenous molecules such as tryptophan and NADH. Since the system optics ...

    keckcenter - 11/17/2015 - 16:14

  7. Papers

    Clinical Applications FLIM Publications ​ FRET Publications Microscopy Publications   ...

    keckcenter - 10/20/2017 - 15:35

  8. Registration

    Follow the link for the 2018 Workshop on FRET and FLIM Microscopy: ... http://artsandsciences.virginia.edu/kcci/workshop/workshop2018/ws_fret_application.php ...

    keckcenter - 05/17/2017 - 10:41

  9. Periasamy, Ph.D.

    and nuclear free calcium in single intact living cells. He developed a 2- and 3-color steady state, confocal ... , multiphoton, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization ... a hands on training Annual Workshop on FLIM & FRET Microscopy at the University of Virginia, ...

    artsci - 10/20/2018 - 19:51

  10. One-day Symposium on Microscopy Imaging at the University of Virginia

    Confocal, Spectral, Multiphoton, in vivo/intravital imaging, TIRF, FRAP, FCS, FLIM, FRET microscopy ...

    keckcenter - 10/01/2018 - 20:07

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