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  1. FLIM-FRET Microscopy

    currently available fluorescence microscopic techniques, such as confocal or multi-photon excitation, cannot ... and frequency domain FLIM imaging systems are available on the market. FLIM-FRET The combination of lifetime ... and FRET (FLIM-FRET) provides high spatial (nanometer) and temporal (nanoseconds) resolution (Bacskai et ...

    keckcenter - 10/20/2017 - 15:24

  2. Multiphoton FRET Microscopy

    and depth resolution without a confocal aperture. The multiphoton technique considerably reduces ... at a wavelength substantially shorter than the excitation wavelength. How to collect FRET Images In Multiphoton ... FRET (MP-FRET) imaging, we select the appropriate filters and high sensitivity photomultiplier tubes ...

    keckcenter - 10/20/2017 - 15:23

  3. FRET Pair Spectra

    FRET Pair Spectra 2-Photon Spectra ...

    keckcenter - 10/20/2017 - 15:25

  4. Zeiss 780 NLO

    Zeiss 780 Confocal/spectral/multiphoton Imaging Microscopy System The Zeiss 780 NLO system ... consists of an inverted Axio Observer microscope, laser scanning confocal, 32 channels spectral imaging, ... the Multi-Timing Macro, Multicolor+3D+time, FRAP, FRET, tile imaging, multitrack scanning to avoid crosstalk, ...

    keckcenter - 10/20/2017 - 16:05

  5. One-day Symposium on Microscopy Imaging at the University of Virginia

    SEM, Confocal, Spectral, Multiphoton, in vivo/intravital imaging, TIRF, FRAP, FCS, FLIM, FRET ...

    keckcenter - 09/18/2015 - 12:59

  6. FLIM Imaging

    photon confocal microcopy, such as less photobleaching, photodamage, scattering for live cell and tissue ... laser scanning microscopes both confocal and multi-photon mode with high speed detectors ... fluorophores such as used for FRET or endogenous molecules such as tryptophan and NADH. Since the system optics ...

    keckcenter - 11/17/2015 - 16:14

  7. Papers

    Clinical Applications FLIM Publications ​ FRET Publications Microscopy Publications   ...

    keckcenter - 10/20/2017 - 15:35

  8. Registration

    Follow the link for the 2018 Workshop on FRET and FLIM Microscopy: ... ...

    keckcenter - 05/17/2017 - 10:41

  9. Periasamy, Ph.D.

    a 2- and 3-color steady state, confocal, multiphoton, and FLIM based Förster resonance energy transfer ... (FRET) imaging system for protein localization in living specimens. He has published over 100 articles ... Sciences through SPIE (since 2001) and runs a hands on training annual workshop  on FLIM & FRET ...

    artsci - 12/09/2017 - 11:06

  10. FLIM Publications

    fluorescnce resonance energy transfer (FRET) and fluorescnce lifetime imaging microscopy (FLIM) have been ... proteins, allow fluorescent resonance energy transfer (FRET) to be used to study protein interactions ... using FRET and FLIM  by Ye Chen, James D. Mills, & Ammasi Periasamy ABSTRACT: Interacting proteins ...

    keckcenter - 10/20/2017 - 16:01