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  1. Widefield FRET Microscopy

    FRET images The wide-field microscopy requires a high-speed and high sensitivity CCD camera (may ... Any fluorescence microscope (inverted or upright) can be converted to use for W-FRET microscopy. ... There are a number of papers listed in the literature for various protein studies using W-FRET systems (Day, 1998; ...

    keckcenter - 10/20/2017 - 15:19

  2. Screening (HCS)

    is used to screen FRET sensors.  Schematic and hardware configuration of the wide-field high content ... on the existing wide-field microscopy system. Two color High-content screening of multi-well plates (Max 96 well ... plates). All the hardware used for the wide-field can be used for the HCS system. Currently HCS system ...

    keckcenter - 10/20/2017 - 16:08

  3. Books

    and tissues, different FRET microscopy systems (wide-field, confocal, multi-photon), FRET data analysis, ... concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance ... energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after ...

    keckcenter - 10/20/2017 - 15:32

  4. FRET

    wide-field,  confocal, spectral and  2p microscopy  as well as  FRET/FLIM, each with its own advantage ... What is FRET? Förster (Fluorescence) Resonance Energy Transfer (FRET) is a process involving ... be higher than or equal to the acceptor fluorophore. There are three important conditions for FRET to occur: ...

    artsci - 10/20/2017 - 15:04

  5. FRET Microscopy

    wide-field,  confocal, spectral and  2p microscopy  as well as  FRET/FLIM, each with its own advantage ... What is FRET? Förster (Fluorescence) Resonance Energy Transfer (FRET) is a process involving ... be higher than or equal to the acceptor fluorophore. There are three important conditions for FRET to occur: ...

    Content Manager - 10/20/2017 - 15:10

  6. Confocal FRET Microscopy

    of brightness and slice thickness. Laser scanning confocal FRET (C-FRET) microscopy overcomes the limitation ... confocal microscopy system for FRET imaging. Disadvantages of LSCM A disadvantage of this technique ... Standard laser lines do allow C-FRET to be used for a number of fluorophore combinations including ...

    keckcenter - 10/20/2017 - 18:55

  7. User Fees

    Wide-field fluorescence microscopy: $15/hour   [Training fee $100- onetime fee per PI] Wide-field ... Micrcoscopy: $40/hour   [Training fee $200- onetime fee per PI] Lifetime imaging (FLIM) and FRET: $40/hour   [Training ... fee $200- onetime fee per PI] Data Analysis Training (FRAP, FRET, FLIM, FCS, Lightsheet, ...

    artsci - 10/20/2017 - 16:11

  8. FRET Pair Spectra

    FRET Pair Spectra 2-Photon Spectra ...

    keckcenter - 10/20/2017 - 15:25

  9. General Information

    lifetime imaging microscopy (FLIM) systems (3), wide-field microscopy, high content screening (HCS) system, ... advanced light microscopy techniques. We run an annual workshop on FLIM & FRET microscopy during March ...

    artsci - 10/20/2017 - 16:04

  10. Papers

    Clinical Applications FLIM Publications ​ FRET Publications Microscopy Publications   ...

    keckcenter - 10/20/2017 - 15:35

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