Methods in Cellular Imaging - Table of Contents

Part I : Basics of Fluorescence, Fluorophores, Microscopy, and Detectors

Introduction, 3

1.    Basics of Fluorescence, 5 - 19 
Keith Berland

2.    Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals, 20 - 39 
Ian S. Harper

3.    Detectors for Fluorescence Microscopy, 40 - 52 
Kenneth R. Spring

4.    Basics of a Light Microscopy Imaging System and its Application in Biology, 53 - 65 
Lance Davidson and Raymond Keller

5.    Laser Scanning Confocal Microscopy Applied to Living Cells and Tissues, 66 -87 
John J. Lemasters, Ting Qian, Donna R. Trollinger, Barbara J. Muller-Borer, Steven P. Elmore, and Wayne E. Cascio

6.    Functional Imaging of Mitochondria Within Cells, 88 - 111 
Michael R. Duchan, Jake Jacobson, Julie Keelan, Mart Mojet, and Olga Vergun

7.    Diffusion measurements by photobleaching recovery methods, 112 - 127 
Alan S. Verkman, Lakshmanan Vetrivel, and Peter Haggie

8.    Processing Microscope-Acquired Images for use in Multimedia, Print and the World Wide Web, 128 - 142 
Harold L. Noakes, Jr. and Ammasi Periasamy


Introduction, 145-146

9.    Basic Principles of multiphoton Excitation Microscopy, 147 - 161 
Peter T.C. So, Ki H. Kim, Christof Buehler, Barry R. Masters, Lily Hsu, and Chen-Yuan Dong

10.    Building a Multiphoton System Using a Laser Scanning Confocal Architecture, 162 - 179 
Alberto Diaspro

11.    Two-Photon Microscopy in Highly Scattering Tissue, 180 - 199 
Vincent P. Wallace, Andrew K. Dunn, Mariah L. Coleno, Bruce J. Tromberg

12.    Multiphoton Laser Scanning Microscopy and Dynamic Imaging in Embryos, 200 - 235 
Mary E. Dickinson and Scott Fraser

13.    In Vivo Diffusion Measurements Using Multiphoton Excited Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy, 216 
Warren R. Zipfel and Watt W. Webb

14.    Cellular Response to Laser Radiation in Fluorescence Microscopes, 236 - 251 
Karsten König


Introduction, 255-256

15.    Measurement of Fluorescence Resonance Energy Transfer in the Optical Microscope, 257 - 272 
Brian Herman, Gerald Gordon, Nupam Mahajan, and Victoria Centonze

16.    Frequency Domain Fluorescence Lifetime Imaging Microscopy: A Window on the Biochemical Landscape of the Cell, 273 - 294 
Peter J. Verveer, Anthony Squire, and Philippe I.H. Bastiaens

17.    Wide-field, confocal, two-photon and lifetime resonance energy transfer imaging microscopy, 295 - 308 
Ammasi Periasamy, Masilamani Elangovan, Horst Wallrabe, Margarida Barroso, James N. Demas, David L. Brautigan, Richard N. Day

18.    One- and Two-photon Confocal lifetime imaging and its applications, 309 - 323 
Hans C. Gerritsen and Klees C. De Grauw

19.    Biological applications of Pump-Probe fluorescence microscopy and spectroscopy in the Frequency Domain, 324 - 339 
Chen Yuan Dong, Christof Buehler, Peter T.C. So, Todd French, and Enrico Gratton


    Introduction, 343-344

    20.    Spectral Microscopy for Quantitative Cell and Tissue Imaging, 345 - 361 
    Daniel L. Farkas

    21.    Total Internal Reflection Fluorescence Microscopy, 362 - 380 
    Daniel Axelrod

    22.    Laser Traps in Cell Biology and Biophysics, 381 - 394 
    William H. Guilford

    23.    Bioluminescence Imaging of Gene Expression In Living Cells and Tissues, 395 - 408 
    Michael E. Geusz

    24.    Imaging Living Cells and Mapping Their Surface Molecules with the Atomic Force Microscope, 409 - 423 
    Muhammed Gad and Atsushi Akai