Error Analysis Of The Rapid Lifetime Determination
Method For Double-Exponential Decays And New
Windowing Schemes
Kristin K. Sharman
ABSTRACT: The rapid lifetime determination method (RLD) is a mathematical technique for extremely rapid evaluations of lifetimes in exponential decays. It has been applied in luminescence microscopy and single-molecule lifetime evaluation....Anal. Chem.1999, 71,947-952
Dynamic Imaging Using Fluorescence Resonance Energy Transfer
Masilamani Elangovan
ABSTRACT: Recently, fluorescnce resonance energy transfer (FRET) and fluorescnce lifetime imaging microscopy (FLIM) have been the buzzwords for monitoring protein-protein interactions in living cells....Biotechniques Vol. 32 No. 6 (2002)
Nanosecond Fluorescence Resonance Energy
Transfer-Fluorescence Lifetime Imaging Microscopy To Localize
The Protein Interactions In A Single Living Cell
Masilamani Elangovan
ABSTRACT: Visualizing and quantifying protein-protein interactions is a recent trend in biomedical imaging. The current advances in fluorescnce microscopy, coupled with the development of new fluorescent probes such as green fluorescent proteins, allow fluorescent resonance energy transfer (FRET) to be used to study protein interactions in living spectrums....Journal Of Microscopy, Vol. 205, Pt 1 January 2002, Pp. 3-14
Fluorescence Lifetime Imaging (FLIM) Of Green Fluorescent Fusion Proteins In Living Cells
Ammasi Periasamy
ABSTRACT: Fluorescence microscopy is an exception al tool for looling inside cells and tissues. Recent advances in fluorescence microscopy, including improved optics, sensitive fluorescent dyes, and high-sensitivity cameras, coupled with technological advances in computes and sophisticated software now permit quantitative measurement and noninvasive acquisition of spectroscopic information from a single living cell....Methods In Molecular Biology, Vol. 183: Green Fluorescent Protein: Applications And Protocals
Time-Gated Fluorescence Microscopy For Clinical Imaging
Assami Periasamy
ABSTRACT: Fluorescence microscopy has been an essential tool for generations of biologists, allowing the examination of details of individual living or fixed cells. The development of a large number of fluorescnece dyes and the ability to attach them to specific molecular cell constituents allows real-time observation and quantification of the activities of these constituents in cells and tissues....USA Microscopy And Analyses, March 1995 19
Time-Resolved Fluorescence Lifetime Imaging Microscopy
Using A Picosecond Pulsed Tunable Dye Laser System
Ammasi Periasamy
ABSTRACT: The design and implementation of a time-resolved fluorescence lifetime imaging microscope (TRFLIM) for the biomedical sciences are described. The measurement of fluorescence lifetimes offers many benefits, among which is that they are independent of local signal intensity and concentration of the fluorophore and they provide visualization of the molecular environment in a single living cell.Rev. Sci. Instrum. 67 (10), October 1996
Protein localization in living cells and tissues using FRET and FLIM
Ye Chen . James D. Mills. Ammasi Periasamy
Abstract: Interacting proteins assemble into molecular machines that control cellular homeostasis in living cells. While the in vitro screening methods have the advantage of providing direct access to the genetic information encoding unknown protein partners, they do not allow direct access to interactions of these protein partners in their natural environment inside the living cell. Using wide-.eld, confocal, or two-photon (2p) .uorescence resonance energy transfer (FRET) microscopy, ..........Differentiation (2003) 71:528–541