Part I : Basics of Fluorescence, Fluorophores, Microscopy, and Detectors
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1. Basics of Fluorescence, 5 - 19
Keith Berland
2. Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals, 20 - 39
Ian S. Harper
3. Detectors for Fluorescence Microscopy, 40 - 52
Kenneth R. Spring
4. Basics of a Light Microscopy Imaging System and its Application in Biology, 53 - 65
Lance Davidson and Raymond Keller
5. Laser Scanning Confocal Microscopy Applied to Living Cells and Tissues, 66 -87
John J. Lemasters, Ting Qian, Donna R. Trollinger, Barbara J. Muller-Borer, Steven P. Elmore, and Wayne E. Cascio
6. Functional Imaging of Mitochondria Within Cells, 88 - 111
Michael R. Duchan, Jake Jacobson, Julie Keelan, Mart Mojet, and Olga Vergun
7. Diffusion measurements by photobleaching recovery methods, 112 - 127
Alan S. Verkman, Lakshmanan Vetrivel, and Peter Haggie
8. Processing Microscope-Acquired Images for use in Multimedia, Print and the World Wide Web, 128 - 142
Harold L. Noakes, Jr. and Ammasi Periasamy
Part II : MULTIPHOTON EXCITATION FLUORESCENCE MICROSCOPY
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9. Basic Principles of multiphoton Excitation Microscopy, 147 - 161
Peter T.C. So, Ki H. Kim, Christof Buehler, Barry R. Masters, Lily Hsu, and Chen-Yuan Dong
10. Building a Multiphoton System Using a Laser Scanning Confocal Architecture, 162 - 179
Alberto Diaspro
11. Two-Photon Microscopy in Highly Scattering Tissue, 180 - 199
Vincent P. Wallace, Andrew K. Dunn, Mariah L. Coleno, Bruce J. Tromberg
12. Multiphoton Laser Scanning Microscopy and Dynamic Imaging in Embryos, 200 - 235
Mary E. Dickinson and Scott Fraser
13. In Vivo Diffusion Measurements Using Multiphoton Excited Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy, 216
Warren R. Zipfel and Watt W. Webb
14. Cellular Response to Laser Radiation in Fluorescence Microscopes, 236 - 251
Karsten König
Part III : FLUORESCENCE RESONANCE ENERGY TRANSFER AND LIFETIME IMAGING MICROSCOPY
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15. Measurement of Fluorescence Resonance Energy Transfer in the Optical Microscope, 257 - 272
Brian Herman, Gerald Gordon, Nupam Mahajan, and Victoria Centonze
16. Frequency Domain Fluorescence Lifetime Imaging Microscopy: A Window on the Biochemical Landscape of the Cell, 273 - 294
Peter J. Verveer, Anthony Squire, and Philippe I.H. Bastiaens
17. Wide-field, confocal, two-photon and lifetime resonance energy transfer imaging microscopy, 295 - 308
Ammasi Periasamy, Masilamani Elangovan, Horst Wallrabe, Margarida Barroso, James N. Demas, David L. Brautigan, Richard N. Day
18. One- and Two-photon Confocal lifetime imaging and its applications, 309 - 323
Hans C. Gerritsen and Klees C. De Grauw
19. Biological applications of Pump-Probe fluorescence microscopy and spectroscopy in the Frequency Domain, 324 - 339
Chen Yuan Dong, Christof Buehler, Peter T.C. So, Todd French, and Enrico Gratton
Part IV. OTHER ADVANCED METHODS IN CELLULAR IMAGING
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20. Spectral Microscopy for Quantitative Cell and Tissue Imaging, 345 - 361
Daniel L. Farkas
21. Total Internal Reflection Fluorescence Microscopy, 362 - 380
Daniel Axelrod
22. Laser Traps in Cell Biology and Biophysics, 381 - 394
William H. Guilford
23. Bioluminescence Imaging of Gene Expression In Living Cells and Tissues, 395 - 408
Michael E. Geusz
24. Imaging Living Cells and Mapping Their Surface Molecules with the Atomic Force Microscope, 409 - 423
Muhammed Gad and Atsushi Akai