Conventional Epifluorescence Microscopy
Olympus (IX-70) Infinity corrected inverted microscope with four output ports.
Microinjection device - Eppendorf; Temperature controlled and perfusion chambers to maintain the viability of the cells/tissue (Bioptech, 1 unit; Medical Systems Corp., 2 units).
Motorized x-, y-, and z-axis system.
Dage MTI color camera and 3-chip cooled Hamamatsu color camera
Slow scan CCD with a back-thinned, back-illuminated imaging chip for luminescence and fluroescence imaging (Cryo CH360, Photometrics).
PXL Photometrics CCD camera (1024x1035, 10bit, 2MHz readout)
Orca-2 (1280x1024, 12/14bit, 10MHz or 1.25MHz readout, ROI selectable) dual scan cooled digital Interline Hamamatsu CCD Camera.
Linux based Inovision software for image acquisition and analysis
Silicon Graphics based Applied Precision DeltaVision software (installed in Silicon Graphics Indigo2 R10000) for digital deconvolution microscopy and three-dimensional reconstruction