Total Internal Reflection Fluorescence (TIRF) Microscopy
- Olympus TIRF system illuminator
- Argon laser (457, 488, 514 nm),HeNe green (543 nm), DPSS (556 nm), HeNe Red (633 nm)
- Arc lamp
- Lude filter wheels (Ex & Em)
- Coolsnap CCD camera (Roper Scientific)
- Metamorph Software
- Temperature controlled chamber and Eppendorf microinjection device
TIRF Imaging on Cell Membrane
TIRF microscopy was used to image GFP-speckle actin in an HT 1080 fibrosarcoma cell (Top) and Alexa488 labeled actin filaments elongation in vitro (Bottom). (Dorothy Schafer, Biology, UVA)
 |
