Nikon Laser Scanning Confocal Microscopy (LSCM)
Nikon PCM2000 laser scanning confocal coupled to TE-200 epifluorescence microscope.
Objective lenses- 4x, 10x, 20x, 20x MI, 40x WI, 60x WI, 100x oil. Phase contrast and DIC Optics.
Two fluorescence channels and one transmission detector.
Lasers - Argon Ion (488 and 457 nm; FITC, GFP, Alexa488, CFP), HeNe Green (543nm; Rhodamine, Cy3, dsRed (RFP)), HeNe Yellow (594nm; Cy5, Texas Red).
Orca-1 (1280x1024, 12bit, 2/4/8 binning, 10MHz readout) Hamamatsu CCD Camera. Excitation and emission filter wheels with shutters and controllers.
Microinjection device - Eppendorf; Temperature controlled and perfusion chambers to maintain the viability of the cells/tissue (Medical Systems Corp.)
The Simple PCI software drives the hardware. This software is used for confocal and camera based image acquisition and processing.
Bio-Rad Radiance 2100 (Conf/2P/FLIM)
Nikon TE300 epifluorescence microscope
Four line argon ion laser, HeNe green and red diode lasers
Coherent 10W Verdi pumped high-speed ti:sapphire pulsed laser system
Software have various functions includes any ROI real time logical operation display, FRAP, physiological measurements, various image analysis functions, reverse scanning to reduce cross talk, Confocal and multiphoton imaging using the same specimen etc., two channel external detectors for multiphoton, four (3 fluorescence + one transmission) internal detectors for confocal and multiphoton
