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Wide-Field
Confocal
Multiphoton
Flim
Software

Two-Photon Excitation Microscope (Home Built)

  • Bio-Rad MRC600 Laser Scanning Confocal Microscope coupled to the Nikon Microscope, TE-300; 20x, 40x, 60x water immersion lenses
  • Femtosecond Modelocked Titanium: Sapphire Laser (Coherent, Inc.) Pulse Width-< 150fs; Repetition Rate - 76 Mhz; Tunable wavelength 720 - 900nm; X-wave Optics; Pumping laser - Verdi (532nm, 5W).
  • Power meter, wavemeter, 4channel oscilloscope, autocorrelator, IR viewer

    Bio-Rad Radiance 2100 (Conf/2P/FLIM)

  • Nikon TE300 epifluorescence microscope
  • Four line argon ion laser, HeNe green and red diode lasers
  • Coherent 10W Verdi pumped high-speed ti:sapphire pulsed laser system
  • Software have various functions includes any ROI real time logical operation display, FRAP, physiological measurements, various image analysis functions, reverse scanning to reduce cross talk, Confocal and multiphoton imaging using the same specimen etc., two channel external detectors for multiphoton, four (3 fluorescence + one transmission) internal detectors for confocal and multiphoton