Filter configurations for confocal image acquisition for selected fluorophore pairs.
| Fluorophore | Excitation wavelengh (nm) | Emission filter (nm) |
| Alexa 488 or GFP | Argon 488 | 515/30 or 535/50 |
| Cy3 or Phod-2 | Green HeNe 543 | 590/70 |
| CFP | Argon 457 | 485/30 |
| dsRED1 | HeNe 543 | 590/70 |
| CFP | Argon 457 | 485/30 |
| YFP | Argon 514 | 528/50 |
| Cy3 | Green HeNe 543 | 590/70 |
| Cy5 | HeNe 633 or HeNe 594 | 660LP |