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Multi-Photon FRET Microscopy

Introduction | How to do FRET | Filters | Example Images

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Filter configurations for multiphoton image acquisition for selected fluorophore pairs.

Fluorophore	Excitation (nm)	Emission (nm)
Alexa 488	790	515/30
Cy3	735	590/70
BFP	740	450/80
eGFP	880	515/30
CFP	820	485/30
YFP	920	528/50
CFP	820	485/30
dsRED1	780	590/70
BFP	740	450/80
S65T (GFP)	770	515/30