CURRICULUM VITAE
AMMASI PERIASAMY, Ph.D.
List of Publications
Guest Editor
Special Issue on Multiphoton Microscopy - Journal of Biomedical Optics, July
2003 issue
Books
“Methods in Cellular Imaging” Oxford University Press, NY, 2001.
“Molecular Imaging:FRET Microscopy and Spectroscopy” Oxford University Press, NY, 2005.
Conference Proceeding Books
“Multiphoton Microscopy in the Biomedical Sciences” SPIE Publications,
Volume 4262, Washington, 2001- present.
Conference Chair (2001-Present)
Multiphoton Microscopy in the Biomedical Sciences, The International Society
for Optical Engineering (SPIE), San Jose, CA.
Workshops
“Interdisciplinary workshop on Advances in Cellulalr Imaging Microscopy”,
1998-1999, seminar series and hands on training for the University of Virginia,
faculty, staffs, and students.
“Workshop on FRET Microscopy”, (International participants), March 9-13, 2002; March 5-9, 2003; March 10-13, 2004; March 8-12, 2005; March 7-11, 2006.
Selected papers from total of 105
Referred Journals
1. Demarco, I. A., Periasamy, A., Booker, C. F. and Day, R. N. (2006) Monitoring dynamic protein interactions with photo-quenching FRET. Nature Methods 3(7):519-524
2. Abadir,, P.M., Periasamy, A., Carey, R.M. and Siragy, H.M. (2006) Angiotension II type 2 receptor-Bradykinin B2 receptor functional heterodimerization. Hypertension (JAHA, Journal of the American Heart Association) 48:1-7.
3. Chen, Y. and Periasamy, A. (2006) Intensity range based quantitative FRET data analysis to localize the protein molecules in living cell nucleus. J. Fluorescence. 16:95-104.
4. Wallrabe, H. Chen, Y., Periasamy, A. And Barroso, M. (2006) Issues in confocal microscopy for quantitative FRET analysis. Microscopy research and Techniques. 69:196-206.
5. Wallrabe, H., and Periasamy, A. (2005) FRET-FLIM microscopy and spectroscopy in the biomedical sciences. Current Opinion in Biotechnology. 16:19-27.
6. Periasamy, A. and Chen, Y. (2005) Quantitative Analysis of Protein-Protein Interactions: One- and Two-photon Excitation FRET-FLIM Microscopy. Microscopy and Microanalysis. 11(suppl 2):12-13.
7. Chen, Y. and Periasamy, A. (2004) Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization. Microscopy Research and Techniques. 63:72-80.
8. Chen, Y., Mills, J.D. and Periasamy, A. (2003) Protein interactions in cells and tissues using FLIM and FRET. Differentiation. 71:528-541.
9. Sekar, R.B. and Periasamy, A. (2003) Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localization. J. Cell Biol., 160:629-633.
10. Solenski, N.J., Kostecki, V.K., Dovery, S. and Periasamy, A. (2003) Nitric-oxide-induced depolarization of neuronal mitochondria:implications for neuronal cell death. Molecular and Cellular Neuroscience. 24:1151-1169.
11. Day, R.N., Voss, T.C., Enwright III, J.F., Booker, C.F., Periasamy, A. and Schaufels, F. (2003) Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBP?) in the living pituitary cell nucleus. Mol. Endo. 17(3), 333-345.
12. Elangovan, M., Wallrabe, H., Chen, Y., Day, R.N., Barroso, M. and Periasamy, A. (2003) Characterization of one- and two-photon excitation resonance energy transfer microscopy. Methods. 29, 58-73.
13. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A. and Barroso, M. (2003) Confocal FRET microscopy to measure clustering of receptor-ligand complexes in endocytic membranes. Biophysical J. 85:559-571.
14. Mills, J.D., Stone, J.R., Rubin, J.D., Melon, D.E., Okonkwo, D.O., Periasamy, A. and Helm, G.A. (2003) Illuminating Protein Interactions in Tissue using Confocal and Two-photon Excitation Fluorescent Resonance Energy Transfer (FRET) Microscopy. J. Biomed. Opt. 8: 347-356.
15. Wallrabe, H., Stanley, M., Periasamy, A. and Barroso, M. (2003) One- and two-photon fluorescence resonance energy transfer microscopy to establish a clustered distribution of receptor-ligand complexes in endocytic membranes. J Biomed. Opt. Vo. 8(3), 339-346.
16. Bowman, R.D., Kneas, K., Demas, J.N. and Periasamy, A. (2003) Conventional, confocal, and two-photon fluoresecnce microscopy investigations of polymer-supported oxygen sensors. J. Microscopy 211:112-120.
17. Elangovan, M., Day, R.N. and Periasamy, A. (2002) Dynamic imaging using fluorescence resonance energy transfer. Biotechniques 32, 1260-1265.
18. Elangovan, M., Day, R.N. and Periasamy, A. (2002) A novel nanaosecond FRET-FLIM microscopy to quantitate the protein interactions in a single living cell. J Microscopy. 205:3-14.
19. Gale, J.E., Meyers, J.R., Periasamy, A. and Corwin, J.T. (2002) Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog’s saccule. J. Neurobiol.50, 81-92.
20. Periasamy, A. (2001) Fluorescence resonance energy transfer microscopy:a mini review. J Biomed. Opt. 6: 287-291.
21. Day, R.N., Periasamy, A. and Schaufele, F. (2001) Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus. Methods. 25: 4-18.
22. Periasamy A, Noakes C, Elangovan M, Keller R, Day R. (2001) MultiPhoton Microscopy and its Applications in the Biomedical Sciences. The Spectrum, Bowling Green University. 14:3: 13-19.
23. Kneas, K. Demas, J., DeGraff, B.A. and Periasamy, A. (2000) Fluorescence microscopy study of heterogeneity in polymer-supported luminescence-based oxygen sensors. Microscopy and Microanalysis. 6: 551-556.
24. Resnick, E.M., Schreihofer, D.A., Periasamy, A. and Shupnik, M.A. (2000) Truncated estrogenreceptor product-1 suppresses estrogen receptor transactivation by dimerization with estrogen receptors ? and ?. J. Biol. Chem., 275: 7158-7166.
25. Stenz, M. and Periasamy, A. (2000) Fluorescence resonance energy transfer microscopy. Microscopy Today, issue#00-3 (April), 10-12.
26. Abraham, A.T., Periasamy, A., Brautigan, D.L. and Hecht, S.M. (2000) Localization of bleomycin in mammalian cells utilizing fluorescence microscopy. J. Natural Products. Cover Page, January –April issues.
27. Chen, S-yu., Periasamy, A., Bing, Y. Herman, B., Jacobson, K. and Sulik, K.K. (2000) Differential sensitivity of mouse neural crest cells to ethanol-induced toxicity. Alcohol 20: 75-81.
28. Periasamy, A., Skoglund, P., Noakes, C. and Keller, R. (1999) An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis. Micros. Res. Tech. 47, 172-181.
29. Sharman, K. K., Demas, J. N., Asworth, H., Snow, N.H. and Periasamy, A. (1999) Error analysis of the rapid lifetime determination (RLD) method for double exponential decays and new windowing schemes. Anal. Chem., 71: 947-952.
30. Periasamy, A. and Day, R.N. (1999) Visualizing Protein Interactions in Living Cells using Digitized GFP Imaging and FRET Microscopy. Meth. Cell Biol., 58: 293-314.
31. Periasamy, A. and Day, R. N. (1998) FRET imaging of Pit-1 protein interactions in living cells. J. Biomed.Opt. 3: 154-160.
32. Herman, B., Wodnicki, P., Kwon, S., Periasamy, A., Gordon, G.W., Mahajan, N., and Wang, X.F. (1997) Recent developments in monitoring calcium and protein interactions in cells using fluorescence lifetime microscopy. J. Fluores., 7: 85-91.
33. Periasamy, A., Wodnicki, P., Wang, X.F., Kwon, S., Gordon, G.W. and Herman, B. (1996) Time resolved fluorescence lifetime imaging microscopy using picosecond pulsed tunable dye laser system. Rev. Sci. Instrum. 67: 3722-3731.
34. Periasamy, A., Wang, X.F., Wodnicki, P., Gordon, G.W., Kwon, S., Diliberto, P.A. and Herman, B. (1995) High speed fluorescence microscopy: Lifetime imaging in the biomedical sciences. J. Micros. Soc. Am., 1: 13-23.
35. Periasamy, A., Siadat-Pajouh, M., Wodnicki, P., Wang, X.F. and Herman, B. (1995) Time-gated fluorescence microscopy in clinical imaging. Micros. Anal., (March) 33-35.
36. Periasamy, A. and Herman, B. (1994) Computerized fluorescence microscopic vision in biomedical sciences. J. Comp. Assist. Micros., 6: 1-26.
37. Siadat-Pajouh, M., Periasamy, A., Ayscue, A.H., Moscicki, A.B., Palefsky, J.M., Walton, L.,DeMars, L.R., Power, J.D., Herman, B., and Lockett, S.J. (1994) Detection of human papillomavirus type 16/18 DNA in cervicovaginal cells by fluorescence based in situ hybridization and automated image cytometry. Cytometry 15: 245-257.
38. Siadat-Pajouh, M., Ayscue, A.H., Periasamy, A. and Herman, B. (1994) Introduction of fast and sensitive fluorescent in situ hybridization method for single copy: Detection of human papillomavirus (HPV) genome. J. Histochem. Cytochem. 42: 1503-1512.
39. Wang, X. F., Periasamy, A., Coleman, D. M. and Herman, B. (1992) Fluorescence lifetime imaging microscopy: Instrumentation and Applications. Critical Rev. Analyt. Chem., 23(5): 369-395.
40. Periasamy, A., Lindroth, J.R. and Thompson, R.P. (1992) Development of a computer-aided video imaging system to study the embryonic chick heart in situ. Innov. Tech. Med. Biol., 13: 30-45.
41. Shoji, Y., Fisher, M.H., Periasamy, A., Herman, B. and Juliano, R.L. (1991) Verapamil and Cycolsporin a modulate Doxorubicin Toxicitin. Cancer Letters. 57: 209-218.
42. Shoji, Y., Akhtar, S., Periasamy, A., Herman, B. and Juliano, R.L. (1991) Mechanism of cellular uptake of modified oligodeoxynucleotides containing methylphosphonate linkages. Nucleic Acid Res. 19: 5543-5580.
43. Chen, E.Q., Lam, C.F. and Periasamy, A. (1991) Effect of refraction on optical microscopic measurement of internal blood-vessel diameter and its correction. J. Micros. 164: 239-245.
44. Periasamy, A. and Singh, M. (1991) Analysis of cardiac displacement pattern as observed on the chest wall by time-average speckle interferometry in health and diseases. Innov. Tech. Med. Biol., 12: 614-638.
45. Periasamy, A., Burns, D.H., Holdren, D. N., Pollack, G. H., and Trombitas, K. (1990) A-Band shortening in single fibers of frog skeletal muscle. Biophys. J., 57: 815-828.
46. Pollack, G.H., Granzier, H.L.M., Mattiazzi, A., Trombitas, K., Periasamy, A. et al (1988) Pauses, steps, and the mechanism of contraction. Adv. Exp. Med. Biol. 226: 617-642.
47. Singh, M., Ramachandran, G. and Periasamy, A. (1986) Laser speckle displacement cardiography. J. Opt., 15: 63-66.
48. Periasamy, A. and Singh, M. (1985) Reconstruction of cardiac displacement patterns on the chest wall by laser speckle interferometry. IEEE Trans. Med. Imaging.,MI-4: 52-57.
49. Singh, M. and Periasamy, A. (1984) Determination of safe exposure levels of He/Ne laser light to the human body by means of erythrocyte irradiation studies. Med. Biol. Engg. Comput., 22: 147-150.
50. Singh, M., Periasamy, A. and Chitra, T. (1982) Development of an optical fiber technique for He-Ne laser screening of human body and its comparison with the integrating sphere method. Med. Biol. Engg. Comput., 20: 111-112.
51. Periasamy, A., Singh, M. and Sivaram, B. M. (1981) Detection of human cardiac activity at apex region by laser speckle. Curr. Sci., 50: 302-304.
52. Periasamy, A., Sivaram, B. M. and Singh, M. and Meyyappan, A. (1979) Measurement of displacement of a curved surface using laser speckle. J. Opt., 8: 12-19.
Book Chapters
53. Periasamy, A., and Chen, Y. (2007) Monitoring the protein interactions in specimens. In: “Methods in Molecular Biology-Fluorescent Proteins: Methods and Applications”, Ed. J. Rothnagel. Humana Press, In Preparation.
54. Day, R.N., Periasamy, A. and Demarco, I. (2006) Imaging protein interactions in living cells using the fluorescent proteins. In: Fluorescent Proteins and their uses. Topics in Fluorescence Spectroscopy, Vol. 12. Eds. C.D. Geddes and J.R. Lakowicz, Springer-Verlag, NY. In Press.
55. Chen, Y., Wallrabe, H. and Periasamy, A. (2006) Förster resonance energy transfer microscopy: Localization of protein molecule in a single living cell. In: Handbook of Biological Nonlinear Optical Microscopy. Eds. P. So and B.R. Masters, Oxford University Press-New York. In Press.
56. Periasamy, A. (2006) Cellular Imaging. In: Encyclopedia of Medical Devices and Instrumentation, (John G. Webster, ed.) Hoboken: John Wiley & Sons, Inc. NY. 2nd edition, Vol. 2, pp. 90-101.
57. Mills, J.D., Stone, J.R., Okonkwo, O.D., Periasamy, A., and Helm, G.A. (2005) Multiphoton FRET microscopy for protein localization in tissue. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 6. 112-125.
58. Chen, Y., Elangovan, M. and Periasamy, A. (2005) FRET data Analysis-The algorithm. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 7. 126-145.
59. Chen, Y. and Periasamy, A. (2005) Time correlated single photon counting (TCSPC) FLIM-FRET microscopy for protein localization. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 13. 239-259.
60. Periasamy, A., Noakes, C., Skoglund, P., Keller, R. and Sutherland, A.E. (2002) Two-photon excitation fluorescence microscopy imaging in Xenopus and transgenic mouse embryos. In: “Confocal and Two-photon Microscopy:Foundations, Applications and Advances”, Ed. A. Diaspro, Wiley-Liss, Inc, NY. pp 271-283.
61. Periasamy, A., Elangovan, M., Elliott, E. and Brautigan, D.L. (2002) Fluorescence lifetime imaging of green fluorescent fusion proteins in living cell. In: “Methods in Molecular Biology, Vol. 183, Green Fluorescent Protein: Applications and Protocols”, Ed. B. Hicks., Humana Press, NJ. pp. 89-100.
62. Noakes, H. and Periasamy, A. (2001) Processing microscope acquired-images for use in multimedia, print and the Worl Wide Web. In: “Methods in Cellular Imaging”, Ed. A. Periasamy, Oxford Univ. Press, NY, pp 128-142.
63. Periasamy, A., Elangovan, M, Wallrabe, H., Demas, J.N., Barroso, M., Brautigan, D.L. and Day, R.N. (2001) Wide-field, Confocal, two-photon and Lifetime resonance Energy Transfer Imaging Microscopy. In: “Methods in Cellular Imaging”, Ed. A. Periasamy, Oxford University Press, NY. Pp295-308.
64. Herman, B., Wang, X.F., Wodnicki, P., Periasamy, A., Mahajan, N., Berry, G. and Gordon, G. (1999) Fluorescence lifetime imaging microscopy. In: Applied Fluorescence in Chemistry, Biology and Medicine. (Eds. B. Strehmel, S. Schrader, H. Seifert and W. Rettig) Springer-Verlag, New York. pp491-507.
65. Wang, X.F., Periasamy, A., Wodnicki, P., Gordon, G.W., and Herman, B. (1996) “Time resolved Fluorescence Lifetime Imaging Microscopy: Instrumentation and Biomedical Applications”, In: Fluorescence Imaging Spectroscopy and Microscopy, Eds. X. F. Wang and B. Herman, Chemical Analysis Series, Vol.137, Wiley & Sons, New York, p 313-350.
66. Periasamy, A. and Singh, M. (1985) Detection of cardiac abnormalities by laser speckle and its comparison with 2D-echocardiography. in: Physiological Fluid Dynamics-1, Eds.S. D. Nigam and M. Singh, SCPFD Publications, I.I.T., Madras, p193-198.
67. Sivaram, B. M. and Periasamy, A. (1985) Velocity profiles of fluid flow by laser speckle interferometry. in: Physiological Fluid Dynamics-1, Eds. S. D. Nigam and M. Singh, SCPFD Publications, I.I.T., Madras, p277-280.
68. Sivaram, B. M., Hemachandran, N., Periasamy, A., and Palanisamy, P. K. (1983) Determination of orthodontic centres of rotation and axes of rotation by laser speckle interferometry. Biomed. Engg. II, Ed. C. William Hall, Pergamon, p17-20.
69. Periasamy, A., Singh, M. and Kalyani, N. (1983) Identification of the cardiac abnormalities by laser speckle pattern as observed on the chest wall. Biomed. Eng. II, Ed. C. William Hall, Pergamon Press, 393-396.